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medium培養基。擴增時的小鼠結腸類器官主要由結腸干細胞和結腸祖細胞組成，分化后的小鼠結腸類器官由結腸吸收細胞、杯狀細胞和少量腸內分泌細胞組成。結腸類器官在自我更新和分化能力、組織結構、細胞類型和功能方面，重現了體內結腸上皮的特征，因此是小鼠結腸研究的理想體外模型。</p> <p>產品型號： </p> <p>所屬分類：培養基</p> <p>更新時間：2023-03-20</p> <p>廠商性質：生產廠家</p> <div class="sgt_btn"><a class="sgt_bl" href="/contact.html" rel="nofollow">聯系我們</a><a class="sgt_br" href="#order">留言詢價</a></div> </div> <div id="clear"></div> </div> <div class="pro_con_tlt"><span>詳情介紹</span></div> <div class="pro_con"><div class="tabledivclass" style="width: 99%; float: left;"><table class="MsoNormalTable ke-zeroborder" border="0" cellspacing="0" align="left" width="702" style="max-width: 748px;"><tbody><tr class="firstRow"><td width="89" valign="center" style=";border: 1px solid windowtext;background: rgb(92, 155, 213)"><p style="margin-bottom: 10px;text-align: center;vertical-align: middle"><strong><span style="font-family: Arial;font-size: 12px">Organoid Kit</span></strong><strong></strong></p></td><td width="100" valign="center" style=";border: 1px solid windowtext;background: rgb(92, 155, 213)"><p style="margin-bottom: 10px;text-align: center;vertical-align: middle"><strong><span style="font-family: Arial;font-size: 12px">Art.No.</span></strong><strong></strong></p></td><td width="257" valign="center" style=";border: 1px solid windowtext;background: rgb(92, 155, 213)"><p style="margin-bottom: 10px;text-align: center;vertical-align: middle"><strong><span style="font-family: Arial;font-size: 12px">Components</span></strong><strong></strong></p></td><td width="107" valign="center" style=";border: 1px solid windowtext;background: rgb(92, 155, 213)"><p style="margin-bottom: 10px;text-align: center;vertical-align: middle"><strong><span style="font-family: Arial;font-size: 12px">Specification</span></strong><strong></strong></p></td><td width="147" valign="center" style=";border: 1px solid windowtext;background: rgb(92, 155, 213)"><p style="margin-bottom: 10px;text-align: center;vertical-align: middle"><strong><span style="font-family: Arial;font-size: 12px">Art.No.</span></strong><strong></strong></p></td></tr><tr><td width="89" valign="center" rowspan="10" style=";border: none"><p style="margin-bottom: 10px;text-align: center;vertical-align: middle"><span><strong>小鼠結腸</strong></span></p></td><td width="100" valign="center" rowspan="5" style=";border: 1px solid windowtext"><p style="margin-bottom: 10px;text-align: center;vertical-align: middle"><span>MA-0817H007L</span></p></td><td width="257" valign="center" style=";border: 1px solid windowtext"><p style="margin-bottom: 10px;text-align: center;vertical-align: middle"><span><strong>小鼠結腸</strong>類器官基礎培養基</span></p></td><td width="107" valign="center" style=";border: 1px solid windowtext"><p style="margin-bottom: 10px;vertical-align: middle"><span>500mL</span></p></td><td width="147" valign="center" style=";border: 1px solid windowtext"><p style="margin-bottom: 10px;text-align: center;vertical-align: middle"><span>MG2204-MC-A500</span></p></td></tr><tr><td width="257" valign="center" style=";border: 1px solid windowtext"><p style="margin-bottom: 10px;text-align: center;vertical-align: middle"><span><strong>小鼠結腸</strong>類器官培養因子B (50x)</span></p></td><td width="107" valign="center" style=";border: 1px solid windowtext"><p style="margin-bottom: 10px;vertical-align: middle"><span>10mL</span></p></td><td width="147" valign="center" style=";border: 1px solid windowtext"><p style="margin-bottom: 10px;text-align: center;vertical-align: middle"><span>MG2204-MC-B500</span></p></td></tr><tr><td width="257" valign="center" style=";border: 1px solid windowtext"><p style="margin-bottom: 10px;text-align: center;vertical-align: middle"><span><strong>小鼠結腸</strong>類器官培養因子C (250x)</span></p></td><td width="107" valign="center" style=";border: 1px solid windowtext"><p style="margin-bottom: 10px;vertical-align: middle"><span>2mL</span></p></td><td width="147" valign="center" style=";border: 1px solid windowtext"><p style="margin-bottom: 10px;text-align: center;vertical-align: middle"><span>MG2204-MC-C500</span></p></td></tr><tr><td width="257" valign="center" style=";border: 1px solid windowtext"><p style="margin-bottom: 10px;text-align: center;vertical-align: middle"><span><strong>小鼠結腸</strong>類器官培養因子D (250x)</span></p></td><td width="107" valign="center" style=";border: 1px solid windowtext"><p style="margin-bottom: 10px;vertical-align: middle"><span>2mL</span></p></td><td width="147" valign="center" style=";border: 1px solid windowtext"><p style="margin-bottom: 10px;text-align: center;vertical-align: middle"><span>MG2204-MC-D500</span></p></td></tr><tr><td width="257" valign="center" style=";border: 1px solid windowtext"><p style="margin-bottom: 10px;text-align: center;vertical-align: middle"><span>EDTA (0.5 M, pH 8.0)</span></p></td><td width="107" valign="center" style=";border: 1px solid windowtext"><p style="margin-bottom: 10px;vertical-align: middle"><span>2mL</span></p></td><td width="147" valign="center" style=";border: 1px solid windowtext"><p style="margin-bottom: 10px;text-align: center;vertical-align: middle"><span>E21912</span></p></td></tr><tr><td width="100" valign="center" rowspan="5" style=";border: 1px solid windowtext"><p style="margin-bottom: 10px;text-align: center;vertical-align: middle"><span>MA-0817H007S</span></p></td><td width="257" valign="center" style=";border: 1px solid windowtext"><p style="margin-bottom: 10px;text-align: center;vertical-align: middle"><span><strong>小鼠結腸</strong>類器官基礎培養基</span></p></td><td width="107" valign="center" style=";border: 1px solid windowtext"><p style="margin-bottom: 10px;vertical-align: middle"><span>100mL</span></p></td><td width="147" valign="center" style=";border: 1px solid windowtext"><p style="margin-bottom: 10px;text-align: center;vertical-align: middle"><span>MG2204-MC-A100</span></p></td></tr><tr><td width="257" valign="center" style=";border: 1px solid windowtext"><p style="margin-bottom: 10px;text-align: center;vertical-align: middle"><span><strong>小鼠結腸</strong>類器官培養因子B (50x)</span></p></td><td width="107" valign="center" style=";border: 1px solid windowtext"><p style="margin-bottom: 10px;vertical-align: middle"><span>2mL</span></p></td><td width="147" valign="center" style=";border: 1px solid windowtext"><p style="margin-bottom: 10px;text-align: center;vertical-align: middle"><span>MG2204-MC-B100</span></p></td></tr><tr><td width="257" valign="center" style=";border: 1px solid windowtext"><p style="margin-bottom: 10px;text-align: center;vertical-align: middle"><span><strong>小鼠結腸</strong>類器官培養因子C (250x)</span></p></td><td width="107" valign="center" style=";border: 1px solid windowtext"><p style="margin-bottom: 10px;vertical-align: middle"><span>0.4mL</span></p></td><td width="147" valign="center" style=";border: 1px solid windowtext"><p style="margin-bottom: 10px;text-align: center;vertical-align: middle"><span>MG2204-MC-C100</span></p></td></tr><tr><td width="257" valign="center" style=";border: 1px solid windowtext"><p style="margin-bottom: 10px;text-align: center;vertical-align: middle"><span><strong>小鼠結腸</strong>類器官培養因子D (250x)</span></p></td><td width="107" valign="center" style=";border: 1px solid windowtext"><p style="margin-bottom: 10px;vertical-align: middle"><span>0.4mL</span></p></td><td width="147" valign="center" style=";border: 1px solid windowtext"><p style="margin-bottom: 10px;text-align: center;vertical-align: middle"><span>MG2204-MC-D100</span></p></td></tr><tr><td width="257" valign="center" style=";border: 1px solid windowtext"><p style="margin-bottom: 10px;text-align: center;vertical-align: middle"><span>EDTA (0.5 M, pH 8.0)</span></p></td><td width="107" valign="center" style=";border: 1px solid windowtext"><p style="margin-bottom: 10px;vertical-align: middle"><span>1mL</span></p></td><td width="147" valign="center" style=";border: 1px solid windowtext"><p style="margin-bottom: 10px;text-align: center;vertical-align: middle"><span>E21911</span></p></td></tr></tbody></table></div><p style="margin-top: 0px;margin-bottom: 10px;padding: 0px;color: rgb(116, 116, 116);font-family: 'Microsoft YaHei', Arial, sans-serif;white-space: normal;background-color: rgb(255, 255, 255)"><strong><span style="font-family: 微軟雅黑;color: rgb(83, 129, 53);font-size: 19px">產品信息</span></strong><strong></strong></p><p style="margin-top: 0px;margin-bottom: 10px;padding: 0px;color: rgb(116, 116, 116);font-family: 'Microsoft YaHei', Arial, sans-serif;white-space: normal;background-color: rgb(255, 255, 255)"><strong></strong></p><p style="margin-top: 0px;margin-bottom: 10px;padding: 0px;color: rgb(116, 116, 116);font-family: 'Microsoft YaHei', Arial, sans-serif;white-space: normal;background-color: rgb(255, 255, 255)"><strong><span style="font-family: 微軟雅黑;color: rgb(83, 129, 53);font-size: 19px">其他自備材料和試劑</span></strong><strong></strong></p><p style="margin-top: 0px;margin-bottom: 10px;padding: 0px;color: rgb(116, 116, 116);font-family: 'Microsoft YaHei', Arial, sans-serif;white-space: normal;background-color: rgb(255, 255, 255)"><span style="font-family: 宋體;line-height: 19px;font-size: 13px">MG&ABW基質膠</span></p><p style="margin-top: 0px;margin-bottom: 10px;padding: 0px;color: rgb(116, 116, 116);font-family: 'Microsoft YaHei', Arial, sans-serif;white-space: normal;background-color: rgb(255, 255, 255)"><span style="font-family: Arial;line-height: 18px;font-size: 12px">上皮類器官基礎培養基 </span></p><p style="margin-top: 0px;margin-bottom: 10px;padding: 0px;color: rgb(116, 116, 116);font-family: 'Microsoft YaHei', Arial, sans-serif;white-space: normal;background-color: rgb(255, 255, 255)"><span style="font-family: 宋體;line-height: 18px;font-size: 12px">類器官消化液</span><span style="font-family: Arial;line-height: 18px;font-size: 12px"> </span></p><p style="margin-top: 0px;margin-bottom: 10px;padding: 0px;color: rgb(116, 116, 116);font-family: 'Microsoft YaHei', Arial, sans-serif;white-space: normal;background-color: rgb(255, 255, 255)"><span style="font-family: 宋體;line-height: 18px;font-size: 12px">潤洗液</span></p><p style="margin-top: 0px;margin-bottom: 10px;padding: 0px;color: rgb(116, 116, 116);font-family: 'Microsoft YaHei', Arial, sans-serif;white-space: normal;background-color: rgb(255, 255, 255)"><span style="font-family: Arial;line-height: 18px;font-size: 12px">類器官冷凍保存培養基(無血清) </span></p><p style="margin-top: 0px;margin-bottom: 10px;padding: 0px;color: rgb(116, 116, 116);font-family: 'Microsoft YaHei', Arial, sans-serif;white-space: normal;background-color: rgb(255, 255, 255)"><span style="font-family: Arial;line-height: 18px;font-size: 12px">DPBS (1X)，液體，不含鈣和鎂</span></p><p style="margin-top: 0px;margin-bottom: 10px;padding: 0px;color: rgb(116, 116, 116);font-family: 'Microsoft YaHei', Arial, sans-serif;white-space: normal;background-color: rgb(255, 255, 255)"><br/></p><p style="margin-top: 0px;margin-bottom: 10px;padding: 0px;color: rgb(116, 116, 116);font-family: 'Microsoft YaHei', Arial, sans-serif;white-space: normal;background-color: rgb(255, 255, 255)"><span style="font-family: Arial;font-size: 12px"> </span><strong><span style=";font-size:19px"><strong>小鼠結腸</strong>類器官complete medium培養基的制備</span></strong></p><p style="margin-top: 0px;margin-bottom: 10px;padding: 0px;color: rgb(116, 116, 116);font-family: 'Microsoft YaHei', Arial, sans-serif;white-space: normal;background-color: rgb(255, 255, 255)"><span style=";font-size:13px">使用無菌操作技術配制<strong>小鼠結腸</strong>類器官的擴增或分化培養基。以配制 10 mL 為例配置<strong>小鼠結腸</strong>類器官擴增或分化培養基，</span></p><p style="margin-top: 0px;margin-bottom: 10px;padding: 0px;color: rgb(116, 116, 116);font-family: 'Microsoft YaHei', Arial, sans-serif;white-space: normal;background-color: rgb(255, 255, 255)"><span style=";font-size:13px">如所需量不同，可相應調整用量。</span></p><p style="margin-top: 0px;margin-bottom: 10px;padding: 0px;color: rgb(116, 116, 116);font-family: 'Microsoft YaHei', Arial, sans-serif;white-space: normal;background-color: rgb(255, 255, 255)"><span style=";font-size:13px">1. 冰上解凍 Mouse Colonic Organoid Supplement B (50x)，Mouse Colonic Organoid Supplement C (250x)和 Mouse</span></p><p style="margin-top: 0px;margin-bottom: 10px;padding: 0px;color: rgb(116, 116, 116);font-family: 'Microsoft YaHei', Arial, sans-serif;white-space: normal;background-color: rgb(255, 255, 255)"><span style=";font-size:13px">Colonic Organoid Supplement D (250x)。</span></p><p style="margin-top: 0px;margin-bottom: 10px;padding: 0px;color: rgb(116, 116, 116);font-family: 'Microsoft YaHei', Arial, sans-serif;white-space: normal;background-color: rgb(255, 255, 255)"><span style=";font-size:13px">2. 注意：各 Supplement 添加物解凍后，建議分別分裝后保存取用，避免反復凍融。</span></p><p style="margin-top: 0px;margin-bottom: 10px;padding: 0px;color: rgb(116, 116, 116);font-family: 'Microsoft YaHei', Arial, sans-serif;white-space: normal;background-color: rgb(255, 255, 255)"><span style=";font-size:13px">3. <strong>小鼠結腸</strong>類器官的擴增培養基：將 200 uL Mouse Colonic Organoid Supplement B (50x)，40 μL Mouse Colonic</span></p><p style="margin-top: 0px;margin-bottom: 10px;padding: 0px;color: rgb(116, 116, 116);font-family: 'Microsoft YaHei', Arial, sans-serif;white-space: normal;background-color: rgb(255, 255, 255)"><span style=";font-size:13px">Organoid Supplement C (250x) 和 40 μL Mouse Colonic Organoid Supplement D (250x) 加至 9.72 mL Mouse</span></p><p style="margin-top: 0px;margin-bottom: 10px;padding: 0px;color: rgb(116, 116, 116);font-family: 'Microsoft YaHei', Arial, sans-serif;white-space: normal;background-color: rgb(255, 255, 255)"><span style=";font-size:13px">Colonic Organoid Basal Medium 中，充分混合，配制成 10 mL <strong>小鼠結腸</strong>類器官擴增培養基。</span></p><p style="margin-top: 0px;margin-bottom: 10px;padding: 0px;color: rgb(116, 116, 116);font-family: 'Microsoft YaHei', Arial, sans-serif;white-space: normal;background-color: rgb(255, 255, 255)"><span style=";font-size:13px">4. <strong>小鼠結腸</strong>類器官的分化培養基：將 200 μL Mouse Colonic Organoid Supplement B (50x)，40 μL Mouse Colonic</span></p><p style="margin-top: 0px;margin-bottom: 10px;padding: 0px;color: rgb(116, 116, 116);font-family: 'Microsoft YaHei', Arial, sans-serif;white-space: normal;background-color: rgb(255, 255, 255)"><span style=";font-size:13px">Organoid Supplement C (250x) 加至 9.76 mL Mouse Colonic Organoid Basal Medium 中，充分混合，配制成 10 mL</span></p><p style="margin-top: 0px;margin-bottom: 10px;padding: 0px;color: rgb(116, 116, 116);font-family: 'Microsoft YaHei', Arial, sans-serif;white-space: normal;background-color: rgb(255, 255, 255)"><span style=";font-size:13px"><strong>小鼠結腸</strong>類器官分化培養基。</span></p><p style="margin-top: 0px;margin-bottom: 10px;padding: 0px;color: rgb(116, 116, 116);font-family: 'Microsoft YaHei', Arial, sans-serif;white-space: normal;background-color: rgb(255, 255, 255)"><span style=";font-size:13px">5. 注意：配制后的<strong>小鼠結腸</strong>類器官擴增或分化培養基可在 2 - 8°C 儲存 10 天。此外，Mouse Colonic Organoid</span></p><p style="margin-top: 0px;margin-bottom: 10px;padding: 0px;color: rgb(116, 116, 116);font-family: 'Microsoft YaHei', Arial, sans-serif;white-space: normal;background-color: rgb(255, 255, 255)"><span style=";font-size:13px">Supplement B(50x)內含有細菌及真菌抗生素(50x)。</span></p><p style="margin-top: 0px;margin-bottom: 10px;padding: 0px;color: rgb(116, 116, 116);font-family: 'Microsoft YaHei', Arial, sans-serif;white-space: normal;background-color: rgb(255, 255, 255)"><br/></p><p style="margin-top: 0px;margin-bottom: 10px;padding: 0px;color: rgb(116, 116, 116);font-family: 'Microsoft YaHei', Arial, sans-serif;white-space: normal;background-color: rgb(255, 255, 255)"><span style=";font-size:13px">8. 將接種完成后的培養板至于 37℃二氧化碳恒溫培養箱中，孵育 15 min 左右待基質膠凝固后取出。</span></p><p style="margin-top: 0px;margin-bottom: 10px;padding: 0px;color: rgb(116, 116, 116);font-family: 'Microsoft YaHei', Arial, sans-serif;white-space: normal;background-color: rgb(255, 255, 255)"><span style=";font-size:13px">9. 配制<strong>小鼠結腸</strong>類器官擴增培養基。</span></p><p style="margin-top: 0px;margin-bottom: 10px;padding: 0px;color: rgb(116, 116, 116);font-family: 'Microsoft YaHei', Arial, sans-serif;white-space: normal;background-color: rgb(255, 255, 255)"><span style=";font-size:13px">10. 待基質膠凝固后，沿孔壁加入提前預熱的<strong>小鼠結腸</strong>類器官擴增培養基，24 孔板每孔 500 μL。</span></p><p style="margin-top: 0px;margin-bottom: 10px;padding: 0px;color: rgb(116, 116, 116);font-family: 'Microsoft YaHei', Arial, sans-serif;white-space: normal;background-color: rgb(255, 255, 255)"><span style=";font-size:13px">11. 將 24 孔板置于 37℃二氧化碳培養箱中培養，待新長出的類器官直徑超過 100 μm 后可進行分化實驗。</span></p><p style="margin-top: 0px;margin-bottom: 10px;padding: 0px;color: rgb(116, 116, 116);font-family: 'Microsoft YaHei', Arial, sans-serif;white-space: normal;background-color: rgb(255, 255, 255)"><span style=";font-size:13px">12. 分化前需要吸去 24 孔板培養孔中的<strong>小鼠結腸</strong>類器官擴增培養基，并加入 500 μL 提前配制的分化培養基，分化</span></p><p style="margin-top: 0px;margin-bottom: 10px;padding: 0px;color: rgb(116, 116, 116);font-family: 'Microsoft YaHei', Arial, sans-serif;white-space: normal;background-color: rgb(255, 255, 255)"><span style=";font-size:13px">通常需要 4 d 以上時間，分化期間每隔 2 d 更換一次分化培養基，分化完成后可進行后續實驗。</span></p><ul style="list-style-type: none;" class=" list-paddingleft-2"><br/></ul><br /> <br /> <div class="pro_con_tlt"><span>留言詢價</span></div> <link rel="stylesheet" type="text/css" href="/css/MessageBoard_style.css"> <script language="javascript" src="/skins/Scripts/order.js?v=20210318" type="text/javascript"></script> <a name="order" id="order"></a> <div class="ly_msg" id="ly_msg"> <form method="post" name="form2" id="form2"> <h3>留言框 </h3> <ul> <li> <h4 class="xh">產品：</h4> <div class="msg_ipt1"><input class="textborder" size="30" name="Product" id="Product" value="小鼠結腸" placeholder="請輸入產品名稱" /></div> </li> <li> <h4>您的單位：</h4> <div class="msg_ipt12"><input class="textborder" size="42" name="department" id="department" placeholder="請輸入您的單位名稱" /></div> </li> <li> <h4 class="xh">您的姓名：</h4> <div class="msg_ipt1"><input class="textborder" 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