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class="prodetail_desc">簡要描述：ABW標準型含酚紅082704基質膠</br>推薦的應用：</br>細胞生長，分化，形態研究，細胞化學功能，細胞遷移/侵襲，細胞基因/蛋白表達。蛋白濃度：8-11mg/ml</br>產品貨號：082704</br>中文名稱：ABW基質膠</br>產品規格：10ml</br>產品品牌：ABW</p> <p>產品型號： 10ml</p> <p>所屬分類：ABW基質膠</p> <p>更新時間：2022-04-11</p> <p>廠商性質：生產廠家</p> <div class="sgt_btn"><a class="sgt_bl" href="/contact.html" rel="nofollow">聯系我們</a><a class="sgt_br" href="#order">留言詢價</a></div> </div> <div id="clear"></div> </div> <div class="pro_con_tlt"><span>詳情介紹</span></div> <div class="pro_con"><p><strong>ABW標準型含酚紅082704基質膠</strong>推薦的應用：</p><p>細胞生長，分化，形態研究，細胞化學功能，細胞遷移/侵襲，細胞基因/蛋白表達。蛋白濃度：8-11mg/ml</p><p>產品貨號：082704</p><p>中文名稱：ABW基質膠</p><p>產品規格：10ml</p><p>產品品牌：ABW</p><p><strong><span><strong>ABW標準型含酚紅082704基質膠</strong></span></strong>可運用領域：</p><p>類器官培養 2.血管生成實驗 3.細胞侵襲實驗，Transwell實驗 4.PDX，CDX模型實驗 5.愿為腫瘤移植，皮下成瘤實驗 6.干細胞培養及細胞分化研究 7.其他待開發實驗類型</p><p> </p><p><img src='https://img72.chem17.com/9/20220307/637822588107292637677.jpg' style='width:800px;'></p><p><img src='https://img72.chem17.com/9/20220307/637822588083580789979.jpg' style='width:800px;'></p><p><img src='https://img72.chem17.com/9/20220307/637822588088884755783.jpg' style='width:800px;'></p><p><img src='https://img73.chem17.com/9/20220307/637822588116297901609.jpg' style='width:800px;'></p><p><img src='https://img73.chem17.com/9/20220307/637822588116297901705.jpg' style='width:800px;'></p><p><img src='https://img73.chem17.com/9/20220307/637822588114737891189.jpg' style='width:800px;'></p><p><img src='https://img73.chem17.com/9/20220307/637822588141258061974.jpg' style='width:800px;'></p><p><img src='https://img73.chem17.com/9/20220307/637822588139698051237.jpg' style='width:800px;'></p><p><img src='https://img73.chem17.com/9/20220307/637822588140010053934.jpg' style='width:800px;'></p><p>082704 ABW基質膠皮下成瘤實驗</p><p>一.實驗材料</p><p>細胞：B16-F10</p><p>培養基：含10?S的*培養基</p><p>耗材：預冷槍頭、預冷離心管</p><p>其他：胰酶，PBS，Matrigengel（ABW貨號：批次a 和批次b 兩個批次）</p><p> </p><p>二. 實驗步驟</p><p>A. 準備基質膠</p><p>1. 4℃解凍Matrigengel，實驗前轉移到冰盒中。（注意：同樣要準備一些4℃預冷的槍頭用于吸取Matrigengel）</p><p>B. 制作細胞懸液</p><p>取對數生長期的細胞，經消化、離心、計數后，用無血清培養基稀釋細胞，制成細胞懸液。分別按1:3和1:6的比例稀釋C品牌、批次a和批次b三種基質膠，并最終調整細胞密度至1×107個/mL。</p><p>C. <img width="1" height="16" src="https://www.chem17.com/UserManage/ueditor/themes/default/images/spacer.gif" word_img="file:///C:\Users\admin\AppData\Local\Temp\ksohtml\wps8233.tmp.png" style="background:url(https://www.chem17.com/UserManage/ueditor/themes/default/images/word.gif) no-repeat center center;border:1px solid #ddd" alt="" />皮下接種細胞</p><p>1. 選取6-8周C57BL/6的雌性小鼠18只，每組3只。</p><p>2. 分別在小鼠后背左右兩側腋下接種1×106個細胞。</p><p>3. 在SPF級屏障環境的條件下飼養14天。</p><p>D. 結果統計</p><p>14天后取出腫瘤，稱取重量。</p><p><img src="https://img72.chem17.com/9/20220307/637822596358978302697.png" style="max-width:800px;max-height:300%;" title="微信圖片_20220307142002.png" alt="" /></p><p><img src="https://img72.chem17.com/9/20220307/637822596360538292394.png" style="max-width:800px;max-height:300%;" title="微信圖片_20220307142006.png" alt="" /></p><p> 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