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class="art-title">還不了解什么是類器官用基質膠？看這里就對了！</h1> <div class="art-info"> 發布時間： 2022-12-15　　點擊次數： 278次 </div> <div class="art-body"> <div>　　<a href="http://www.51player.com/Products-36775610.html" target="_blank">類器官用基質膠</a>在4°C條件下為液態，但在加熱到37°C時呈凝膠狀態?；|膠凝固后，重新放回4°C過夜，基質膠可再次液化。(小提示：基質膠液化，建議將基質膠放4°C 冰箱的冰盒里，保持溫度穩定，基質膠液化完)。適用于類器官生長、分化、代謝和毒理學研究，體內和體外血管生成實驗和免疫缺陷小鼠成瘤實驗。<br /> </div><div>　　類器官(organoids)是一種利用具有干性潛能的細胞體外培養出的3D細胞培養物。由于與對應的器官擁有高度相似的組織學特征，具有自我更新和自我組織能力，并能重現該器官的部分生理功能，因此類器官可作為多種疾病的體外模型，在干細胞與發育、再生醫學、疾病研究、藥物開發和精準醫療等多個方面擁有廣泛的應用前景。類器官在體外培養時，由于周期較長，培養過程中注意事項較多，操作較為繁瑣，在操作過程中難免會遇到些許"疑難雜癥"導致實驗失敗。<br /> </div><div>　　如何選擇構建類器官的來源細胞？<br /> </div><div>　　答：理論上所有類器官都可以選擇從ESC/IPSC來構建，部分類器官可以選擇從組織中分離成體干細胞來構建，成體干細胞來源的類器官其構建周期短，體外傳代次數多，相對而言構建成本低；根據實驗需求，如果要通過類器官模型來研究器官的發育，需要對起始細胞進行基因編輯，一般都是通過ESC/IPSC來構建類器官。<br /> </div><div>　　注意事項：<br /> </div><div>　　類器官用基質膠在溫度高于10°C時就會開始凝固成膠，所以盡量在冰上操作基質膠。類器官傳代時，如果為了避免酶對類器官造成影響，可直接用4°C預冷的基礎培養基對基質膠進行緩慢吹打，即可將類器官從基質膠中釋放出來。</div> </div> <div class="art-next"> <dl 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