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class="art-body"> 　　低濃度基質膠是通過基因編輯技術將多種表達膠原蛋白的基因序列插入到經永生化處理的小鼠子宮肌瘤細胞系中，并在實體腫瘤中抽提出來。其主要成分有層粘連蛋白、IV型膠原、巢蛋白、硫酸酐素糖蛋白，還包含生長因子和基質金屬蛋白酶等。<div>　　</div><div>　　一、基質膠用途？</div><div>　　1、Matrigel有利于體外細胞的培養和分化；</div><div>　　2、可用于對細胞形態、生化功能、遷移、侵染和基因表達等的研究；</div><div>　　3、提高人腫瘤細胞在裸鼠上的生長速度；</div><div>　　4、建立評估腫瘤細胞侵襲能力的模型。</div><div>　　基質膠是從富含胞外基質蛋白EHS小鼠腫瘤中提取出的可溶性基底膜制備物，其主要成分由層粘連蛋白，Ⅳ型膠原，硫酸乙酰肝素蛋白聚糖（HSPG）和巢蛋白等組成，還包含生長因子如TGF-beta、EGF、IGF、FGF、組織纖溶酶原激活物和EHS腫瘤自身含有的其他生長因子。</div><div>　　</div><div>　　二、低濃度基質膠特征：</div><div>　　敏感性：對溫度十分敏感；</div><div>　　成膠性：在常溫下快速形成膠狀物；</div><div>　　三維結構：可聚合形成具有生物活性的三維膠質結構；</div><div>　　顏色變化：具有有色差變化（淡黃色到深紅色）；</div><div>　　用途廣：可模擬體內細胞基底膜的結構、組成、物理特性和功能；</div><div>　　適用性強：細胞可在0.5mm-1mm厚度的基質層表面生長。</div><div>　　</div><div>　　三、薄膠成膠方法：</div><div>　　1、凍融后，用預冷的移液槍頭混勻Matrigel基質成勻漿狀。</div><div>　　2、將需要使用的培養板置于冰上，加入濃度為50μL/cm2生長面積的Matrigenge|基質。</div><div>　　3、在37°C放置30分鐘，即可使用。</div> </div> <div class="art-next"> <dl class="shangxia"> <dd>上一篇：<a href="/News-1448104.html">還不了解什么是類器官用基質膠？看這里就對了！</a></dd> 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