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class="art-info"> 發布時間： 2022-08-22　　點擊次數： 288次 </div> <div class="art-body"> 　　<a href="http://www.51player.com/Products-36775483.html" target="_blank">082721基質膠</a>是分離自小鼠腫瘤的基底膜基質，富含包外基質蛋白如層粘連蛋白、IV型膠原、硫酸肝素糖蛋白等，不同黏度(cPs)的甲基纖維素在相同濃度下的流動性也不同，相同濃度下充分溶脹的甲基纖維素流動性與其黏度成反比。<div>　　它是從富含胞外基質蛋白的EHS小鼠腫瘤中提取出基底膜基質，其主要成分有層粘連蛋白、Ⅳ型膠原、巢蛋白、硫酸肝素糖蛋白，還包含生長因子和基質金屬蛋白酶等。</div><div>　　</div><div>　　在室溫條件下，Matrigel聚合形成具有生物學活性的三維基質，模擬體內細胞基底膜的結構、組成、物理特性和功能，有利于體外細胞的培養和分化，可用于對細胞形態、生化功能、遷移、侵染和基因表達等的研究。</div><div>　　</div><div>　　082721基質膠的用途？</div><div>　　1、Matrigel有利于體外細胞的培養和分化；</div><div>　　2、可用于對細胞形態、生化功能、遷移、侵染和基因表達等的研究；</div><div>　　3、提高人腫瘤細胞在裸鼠上的生長速度；</div><div>　　4、建立評估腫瘤細胞侵襲能力的模型。</div><div>　　 </div><div>　　薄膠成膠方法:</div><div>　　1、凍融后，用預冷的移液槍頭混勻Matrigel基質成勻漿狀。</div><div>　　2、將需要使用的培養板置于冰上，加入濃度為50μL/cm2生長面積的Matrigenge|基質。</div><div>　　3、在37°C放置30分鐘，即可使用。</div><div>　　為了保證基質膜的成膠性能與穩定性，稀釋濃度不應該參考不同批次的COA報告中產品濃度確定，一般在1:1-1:2之間，可用無血清培養基稀釋，Matrigenel成膠后立即使用。</div> </div> <div class="art-next"> <dl class="shangxia"> <dd>上一篇：<a 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