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class="art-info"> 發布時間： 2022-07-20　　點擊次數： 362次 </div> <div class="art-body"> 　　<a href="http://www.51player.com/Products-36775198.html" target="_blank">matrigel基質膠</a>是從富含胞外基質蛋白的EHS小鼠腫瘤中提取出基底膜基質，其主要成分有層粘連蛋白、Ⅳ型膠原、巢蛋白、硫酸肝素糖蛋白，還包含生長因子和基質金屬蛋白酶等。它會有色差變化（淡黃色到深紅色），是由于酚紅和碳酸氫鹽與CO2的作用引起的，但是與5%CO2平衡后色差即會減少。<div>　　</div><div>　　1、基質膠凍融操作：</div><div>　　收貨后，若不立刻使用基質膠，應馬上將仍然是冷凍固態的基質膠放進-20℃冰箱保存。使用前須先將基質膠凍融。</div><div>　　</div><div>　　2、凍融：</div><div>　　將matrigel基質膠埋于鋪滿碎冰的冰盒中，然后將整冰盒放入4℃冰箱stayovernight溶解。（普通濃度的基質膠，溶解時間1-2天；高濃度基質膠溶解時間3-7天）。</div><div>　　</div><div>　　3、分裝：</div><div>　　溶解后的須分裝保存，以避免反復凍融。液態基質膠，會在10℃以上快速成膠（特別是高濃度基質膠），因此，分裝時，接觸到基質膠的耗材必須預冷（如移液管、吸頭、EP管等），并且整個實驗必須無菌、冰上操作。（高濃度的基質膠比較粘稠，用移液器不好吸取，可以使用去掉針頭的預冷注射器吸取。）</div><div>　　</div><div>　　4、保存：</div><div>　　分裝后的基質膠在冰上應仍然呈現液態，此時將基質膠放進-20℃保存即可。進行實驗時，取出分裝好的小管，4℃凍融。若分裝好后基質膠已經凝固，說明分裝操作不能很好地保持低溫，導致基質膠已經成膠，不適宜使用。</div><div>　　</div><div>　　5、普通濃度基質膠操作：</div><div>　　細胞可在0.5mm厚度的基質膠表面生長，可以在1mm厚度的三維基質內生長。過度稀釋的基質膠會形成非膠質的蛋白層，可以用于細胞貼壁，但不能用于細胞的研究分化。為保證基質膠的成膠性能與穩定，稀釋濃度不應低于1：3，可用預冷無血清培養基稀釋，成膠后立即使用。</div> </div> <div class="art-next"> <dl class="shangxia"> <dd>上一篇：<a 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