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<div class="art-info"> 發布時間： 2022-09-06　　點擊次數： 1105次 </div> <div class="art-body"> 　　<a href="http://www.51player.com/ParentList-2335935.html" target="_blank">高濃度ABW基質膠</a>是通過基因編輯技術將多種表達膠原蛋白的基因序列插入到經永生化處理的小鼠子宮肌瘤細胞系中，并在實體腫瘤中抽提出來。其主要成分有層粘連蛋白、IV型膠原、巢蛋白、硫酸酐素糖蛋白，還包含生長因子和基質金屬蛋白酶等?？蓪崿F進口品牌基質膠的國產替代。<div>　　</div><div>　　1、高濃度ABW基質膠的使用</div><div>　?、倩|膠顏色的變化可能發生在冷凍或解凍ABW®Matrigengel基底膜基質藥瓶的過程中，由于二氧化碳與重碳酸鹽緩沖液和酚紅的相互作用，顏色會從淡黃色變化到深紅色。顏色的變化是正常的，②不影響產品功效，在與5%的二氧化碳平衡后顏色將消失。</div><div>　　在冰上4°C解凍ABW®Matrigengel基底膜基質。一旦解凍，旋轉藥瓶以確保材料均勻分散。向藥瓶的頂端噴灑70%乙醇，并在空氣中干燥。將產品放置在冰上，處理時請使用無菌操作。利用預冷的移液管、吸頭和管分裝材料，并立即冷凍。避免反復凍融。</div><div>　　</div><div>　　2、預防措施</div><div>　　ABW®Matrigengel基底膜基質在22°C到35°C將快速凝膠化。請在冰上4°C過夜解凍。在使用前請將產品放置在冰上，當準備使用ABW基質膠時，請預冷移液管、吸頭和管。</div><div>　　</div><div>　　3、包被程序</div><div>　　注意：一旦凝膠化，ABW®Matrigengel基底膜基質應該被立即使用。我們推薦至少6mg/mL蛋白濃度的ABW®Matrigengel基底膜基質。ABW®Matrigengel基底膜基質的濃度是批次特異的，并且以分析證明書為基礎。您可以預先檢查基質的批次，通過聯系ABW來預定適合您蛋白濃度的特定批次的ABW®Matrigengel基底膜基質。</div><div>　　按推薦的方法解凍ABW®Matrigengel基底膜基質。使用預冷的移液管，均勻的混合。</div><div>　　將24孔細胞培養板放置在冰上，向每個孔中加入0.289mL冷凍的ABW®Matrigengel基底膜基質(10mg/mL)。該量對于覆蓋整個生長表面應該是足夠的。如果ABW®Matrigengel基底膜基質需要稀釋到10mg/mL，請使用無血清培養基稀釋。</div><div>　　在移液管吸取液體加入到每個孔中時，請避免氣泡。如果孔中含有氣泡，請在4°C預冷的離心機中將培養板離心300xg，10分鐘。</div> 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