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<div class="art-info"> 發布時間： 2022-08-11　　點擊次數： 401次 </div> <div class="art-body"> 　　<a href="http://www.51player.com/Products-36775297.html" target="_blank">082706基質膠</a>的主要成分是層粘連蛋白、IV型膠原蛋白、巢蛋白和硫酸乙酰肝素蛋白多糖，具有低內毒素、低DNA含量的優勢，主要用于支持體外細胞生長和分化、代謝/毒理學研究、細胞黏附、血管新生、細胞遷移/侵襲和體內成瘤實驗等。<div>　　</div><div>　　一、基質膠用途：</div><div>　　1、有利于體外細胞的培養和分化；</div><div>　　2、可用于對細胞形態、生化功能、遷移、侵染和基因表達等的研究；</div><div>　　3、提高人腫瘤細胞在裸鼠上的生長速度；</div><div>　　4、建立評估腫瘤細胞侵襲能力的模型。</div><div>　　將本品基質膠小瓶從-20℃冰箱轉移到4℃冰箱（冰上）過夜解凍（蛋白濃度高時可能需要更多時間），待液化后用渦旋振蕩小瓶以確?；|膠分散均勻。如一次用不完，可冰上分裝一次，僅可反復凍融一次。如基質膠有較多氣泡，可400g低溫離心5分鐘加以去除。將基質膠放置在無菌區域，冰上待用。（凝膠階段注意不要使基質膠過度干燥；融化后的基質膠在10℃以上會逐漸凝固，整個實驗過程需始終保持基質膠置于冰上）。</div><div>　　</div><div>　　二、薄膠成膠方法：</div><div>　　1、凍融后，用預冷的移液槍頭混勻Matrigel基質成勻漿狀。</div><div>　　2、將需要使用的培養板置于冰上，加入濃度為50μL/cm2生長面積的Matrigenge|基質</div><div>　　3、在37°C放置30分鐘，即可使用。</div><div>　　</div><div>　　三、注意事項：</div><div>　　1、082706基質膠的充分融化至少需要4℃過夜，某些情況下，產品濃度很高，則需要更久的時間來融膠；</div><div>　　2、整個操作過程中，所有接觸基質膠的培養器皿，耗材和培養液均應預冷，因為基質膠在超過10℃的情況下就會開始成膠；</div><div>　　3、質膠的使用濃度不要低于3mg/ml。體內實驗為了防止不充分成膠，基質膠的濃度不要低于4mg/ml，成膠最佳濃度為6-8mg/ml；</div><div>　　4、為了您的安全和健康，請穿實驗服并戴一次性手套操作。</div> </div> <div class="art-next"> <dl 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