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<div class="art-info"> 發布時間： 2022-07-07　　點擊次數： 403次 </div> <div class="art-body"> 　　<a href="http://www.51player.com/Products-36864683.html" target="_blank">無外泌體胎牛血清</a>用外泌體研究，無支原體，無內毒素。胎牛血清（FetalBovineSerum,FBS）是大多數細胞培養重要的生長支持物。天然來源的FBS會含有大量的外泌體（exosome）等囊泡（ExtracellularVesicles）成分。由于FBS來源的外泌體會對研究結果產生巨大干擾，因此，外泌體研究時必須使用去除外泌體后的FBS進行細胞培養。<div>　　</div><div>　　細胞在正常含血清的培養基中培養一定的時間后，細胞融合度約為60%-70%時，移去原有含血清的培養基，換成新鮮的無外泌體胎牛血清，繼續培養48h左右，細胞融合度達到80%-95%左右時收取上清,該上清液即可用于提取外泌體。</div><div>　　</div><div>　　對于大多數細胞來說，體外培養時所用的標準培養基都必須在基礎培養基的基礎上外加FBS作為生長添加物。常用的FBS通常來至牛血清，其中含有大量的牛外泌體。這些外泌體含牛的相關蛋白質或miRNA等分子，當研究相關細胞自身分泌外泌體時，牛血清中的外泌體可能會造成嚴重的背景問題，從而影響或干擾結果的判斷。本品專為外泌體研究而設計，由無菌采集健康胎牛血清過濾而成，去除97%以上的胎牛血清內源外泌體。具有無支原體，無內毒素等特點。</div><div>　　</div><div>　　注意事項：</div><div>　　1、本品在凍存之后高豐度蛋白會形成結晶，在NTA檢測時會有雜峰出現，此屬正?，F象。</div><div>　　2、本品已經過無菌處理，可直接用于細胞培養。使用前請將本產品放置于2-8℃自然解凍，或分裝后于-20℃凍存，但請勿多次反復凍融。</div><div>　　3、使用本品，請勿進行高溫熱滅活。</div> </div> <div class="art-next"> <dl class="shangxia"> <dd>上一篇：<a 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